vibrio strains  (ATCC)

Journal: Frontiers in Immunology

Article Title: Carvacrol as a one health–relevant antimicrobial agent: mechanistic insights on its impact on gnotobiotic Artemia and Vibrio campbellii interactions

doi: 10.3389/fimmu.2026.1797445

Figure Lengend Snippet: Impact of carvacrol on the survival of Artemia nauplii after 48h challenge with V. campbelli BB120. Error bars represent the standard error of five replicates. Different letters indicate significant differences (one-way ANOVA with Tukey’s post hoc test; P < 0.05). “Run 1” and “Run 2” represent two independent repetitions of the same challenge experiment, conducted to confirm the reproducibility of the results.

Article Snippet: Vibrio campbellii strain BB120 (= ATCC BAA-1116), stored at 40% glycerol at -80 °C, was first grown in Luria Bertani (LB) agar (Difco Laboratories, Detroit, MI, USA) prepared by supplementing 35 g L -1 of Instant Ocean (LB 35 ).

Techniques:

Journal: Frontiers in Immunology

Article Title: Carvacrol as a one health–relevant antimicrobial agent: mechanistic insights on its impact on gnotobiotic Artemia and Vibrio campbellii interactions

doi: 10.3389/fimmu.2026.1797445

Figure Lengend Snippet: Impact of carvacrol on the growth of V. campbellii BB120 in LB medium containing 35 g/L of synthetic sea salt. Error bars represent the standard error of 3 replicate cultures.

Article Snippet: Vibrio campbellii strain BB120 (= ATCC BAA-1116), stored at 40% glycerol at -80 °C, was first grown in Luria Bertani (LB) agar (Difco Laboratories, Detroit, MI, USA) prepared by supplementing 35 g L -1 of Instant Ocean (LB 35 ).

Techniques:

Journal: Frontiers in Immunology

Article Title: Carvacrol as a one health–relevant antimicrobial agent: mechanistic insights on its impact on gnotobiotic Artemia and Vibrio campbellii interactions

doi: 10.3389/fimmu.2026.1797445

Figure Lengend Snippet: Impact of carvacrol on (A) biofilm formation, (B) hemolytic activity, (C) caseinase activity, (D) lipase activity, and (E) phospholipase activity of V. campbellii BB120. The activities of different virulence-related enzymes were expressed as the ratio between the activity zone and colony diameter. Error bars represent the standard error of three replicates. Asterisks indicate a significant difference when compared with non-treated BB120 (independent samples t -test; NS, not significant; * P < 0.1, ** P < 0.01).

Article Snippet: Vibrio campbellii strain BB120 (= ATCC BAA-1116), stored at 40% glycerol at -80 °C, was first grown in Luria Bertani (LB) agar (Difco Laboratories, Detroit, MI, USA) prepared by supplementing 35 g L -1 of Instant Ocean (LB 35 ).

Techniques: Activity Assay

Journal: Frontiers in Immunology

Article Title: Carvacrol as a one health–relevant antimicrobial agent: mechanistic insights on its impact on gnotobiotic Artemia and Vibrio campbellii interactions

doi: 10.3389/fimmu.2026.1797445

Figure Lengend Snippet: Impact of carvacrol (6.6 µM) treatment on the expression of (A) hsp70 , (B) proPO , (C) ftn and (D) tgase genes in Artemia nauplii at 6, 12 and 24 h after challenge with Vibrio campbellii BB120. Error bars represent the standard errors of three biological replicates. Within each time point, bars sharing the same letters are not significantly different, whereas different letters indicate significant differences (one-way ANOVA with Tukey’s post hoc test; P < 0.05). Lowercase letters refer to 6 h, uppercase letters to 12 h, and asterisk letters to 24 h post-challenge time point.

Article Snippet: Vibrio campbellii strain BB120 (= ATCC BAA-1116), stored at 40% glycerol at -80 °C, was first grown in Luria Bertani (LB) agar (Difco Laboratories, Detroit, MI, USA) prepared by supplementing 35 g L -1 of Instant Ocean (LB 35 ).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Revisiting bioluminescence and sucrose utilization in aquatic pathogens Vibrio harveyi and V. campbellii using genome-wide in silico mapping and phenotyping

doi: 10.1038/s41598-026-37651-3

Figure Lengend Snippet: Gel picture showing presence of luxA ( A and B ) and scrA ( C and D ) gene. ( A ) and ( B ): M, 100 bp ladder; L1 to L18 represents 18 V . harveyi strains (L1, ATCC 14126; L2, LMG 7890; L3, LMG 19643; L4, SB1; L5, V2301; L6, V2426; L7, V2427; L8, V2432; L9, V2437; L10, V2438; L11, V2439; L12, V2441; L13, V2452; L14, Y/12–23; L15, Y2/12–23; L16, FCD2023/4; L17, FCD2023/7; L18, FCD2025/2) L19, Negative control for V. harveyi ; L20 to L50 represents 31 V . campbellii strains (L20, LMG11216/CAIM 519 T; L21, BAA-1116; L22 to L50, 29 strains between LB1 to LB516; L51, Negative control for V. campbellii ). ( C )and ( D ): Distribution of scrA gene. M; 100 bp ladder; L1 to L18 represents 18 V . harveyi strains having desirable 698 bp amplicon. L19, Negative control for V. harveyi ; L20 to L50 represents 31 V . campbellii strains with missing scrA gene. L51: Positive control for scrA using V. alginolyticus . L52: Negative control for V. campbellii

Article Snippet: Of these lux-positive strains, only the type strain ATCC 14126 was available for phenotypic testing, and it exhibited luminescence consistent with its genomic profile (Fig. B).

Techniques: Negative Control, Amplification, Positive Control